الخبرة العملية
Polymerase Chain Reaction : ( PCR, RT-PCR, Long PCR, Multiplex PCR,RFLP
Cell culture
Microbiology Quality Control - Food & Beverage Analysis
Flow cytometry
Lab Safety Rules, Metric System Prefixes, Molarity and Solutions, Lab Biosecurity &Biosafety
Western, Southern, and Northern blotting
SDS-PAGE, Native-PAGE
2D gel analysis and quantification for proteins
Restriction Digestion and Making an Agarose Gel
Working with raw sequence data
cDNA synthesis& transformation,
DNA cloning and purification
Genetic engineering, in vitro mutagenesis,
Restriction digestion, Restriction analysis studies
Ligation of DNA fragments to the vector DNA,
ELISA
Hybrid Assay
Cell culture
Gene prediction analysis
Bioinformatics programs & databanks skills:
Sequence analysis by DNASTAR lagergene software, chromas, bioedit
Sequence alignment techniques (single and multiple) and programs (BLAST,
Clustal, TreeView X 0.5.0, iCODEHOP, Molsoft).
Phylogenetic analysis of DNA sequences; Phylogenetic tree construction (PHYLIP, MEGA, UPGMA, etc.); Evolutionary distance calculations
Gene and protein structure analysis
Genbank, EMBL, UniProt, SWISS-PROT EXPASY
Genetic engineering & cloning software (PDRAW32, CLONEWIN, DNASTAR )
Sequence Scanner v1.0
Next-Generation Sequencing Data AnalysisPrimer Design
DESeqversion 1.9.12,
GenBank Submission(, BankIt, Sequin)and GISAID outbreak.info
