Professional Experience
* Research interest:
Molecular Medicine, Molecular Biology, Medical Physiology, Oncology, Biotechnology, Cell Biology, Biochemistry, and Genetics.
* Medical and Molecular techniques and methods:
o PCR: qPCR, RT-PCR, and conventional PCR, PCR optimization.
o Immunoblotting: Western blotting, ELISA, dot-blot.
o Immunoprecipitation: Chromatin immunoprecipitation (Chip-Seq and Chip-qPCR), RNA immunoprecipitation (RIP-seq and RIP-qPCR), Co-immunoprecipitation (Co-IP) followed by WB or Mass spectrometry, and Crosslinking immunoprecipitation followed by sequencing (CLIP-seq).
o Immunostaining: Immunocytochemistry
o Cell and Tissue culture: cell culture of adherent and suspension cells, cell passages, and cryopreservation.
o Assays: Wound healing, migration, invasion, and proliferation assay.
o Primer and Probe designing
o Extraction methods: Nucleic acid extraction (Eukaryotic, prokaryotic, and gel) and protein extraction (Cytoplasmic and Nuclear)
o Protein determination: BCA assay, Bradford assay, and Qubit 4 based determination)
o Transfection (lipofectamine and electroporation) and transformation (heat shock).
o Gene knockdown (RNA silencing)
o Gene knock-in (plasmid insertion)
o Site-directed mutagenesis (generate mutation)
o Electrophoresis: PAGE, Agarose Gel Electrophoresis.
o Staining gel and blots: Silver staining, Ponsue Red, BradFord, and Coomassie Blue.
o Microscope: Confocal Microscope (nondependent), widefield fluorescence microscope, Light Microscope, Dark-field Microscopy, Dissecting Microscope.
o Others: Sonication (Eukaryotic and Prokaryotic), Spectrophotometer, Nanodrop, Plate reader, Autoclaving, ...etc.
* Medical and Molecular software:
o Creation of Image, Sketch and Diagram: Biorender, Adobe Photoshop, IBS, GraphPad prism, and Excel.
o Statistical analysis: GraphPad prism
o Sequencing analysis: KEGG, Venn graph, Reactome, and Heatmap.
o Image analysis software: ImageLab (Western blot), ImageJ, GraphPad, and PhotoScape... etc.
o Gene manipulation and Primer design software: Snap gene and Serial Cloner
o PCR software: QuantStudio™ Design & Analysis Software (3, 5, and 6 pro) and Step One Software v2.3
o Confocal Microscope: ZEN
o Quality and quantity detection software: Nanodrop and Plate reader software
o Referencing software: Mendeley and RefWorks.
o Excel: for Analyzing NGS data, qPCR fold enrichment, RIP-seq, Chip-seq fold enrichment.
* Medical and Biological Website:
o Signaling pathway analysis: KEGG and Reactome
o Alignment Tools: NCBI nucleic acid BLAST and protein BLAST
o Oligo (primer and probe) designing tools and calculation for PCR, qPCR, cloning, and Sanger sequencing: Primer-BLAST, Primer3, ThermoCalc. and RC, OligoPerfect.
o Molarity, Mass, OD, and dilution calculation: NEBio and GraphPad
o RNA Structure Prediction: ViennaaRNA Web Services.
o Protein-Protein interaction and connection: STRING
o Bioinformatics: SMS, Venn graph, dosztanyi web, Phobius, Biobam, MPI, GOrilla, RCSB, PhosphositePlus, and IUpred.
o Sketch and Diagram design website: Biorender
